Duke Ellington Biography Essay Example | Topics and Well Written Essays - 250 words

Duke Ellington Biography - Essay Example However, he began to attend concerts and was enthralled by the work of ragtime pianists and from here he began working on his musical style. In 1917, Duke Ellington began his musical career by playing the piano at night. He formed a band, and the drummer of this band later felt it was necessary to move to New York City to join a local orchestra. Duke Ellington followed the drummer, moving to Harlem and becoming an influential figure here, perhaps more so than he had been at home in Washington D.C. In 1924, Duke Ellington made his first eight records, and in 1925 began contributing to the Chocolate Kiddies revue, which was designed to introduce people to African American music. From here, his career really began to bloom. Duke Ellington became very famous, playing a number of important jazz clubs and even being nominated for a Pulitzer Prize. However, unfortunately, he died of lung cancer at the age of 75. Over 12,000 people attended his funeral, including his widow and Ella Fitzgerald, another influential jazz musician of the time. Since his death, Duke Ellington has continued to be recognized as one of the most important figures in 20th-century American music and continues to have an influence over jazz and popular music today.

Widespread Use Of Digital Media Media Essay

Widespread Use Of Digital Media Media Essay This essay will critically asses the impact that widespread use of digital media has on broader culture and society. For me, this is questioning whether digital media, the technological revolution and the rise of the internet can be seen as a blessing or a curse on culture and society. According to Castells (2002) who writes avidly on this topic, new media technologies simultaneously reinforce relations of cultural capital, hierarchy and distinction, while enabling social movements to publicise campaigns and connect with distant others. Technology, the internet and the digital media has created unimaginable wealth yet also encouraged millions to work for nothing. Digital media Challenges authority yet allowed regimes to spy and censor as never before. The internet opens up new realms of knowledge and Al Gore (former vice president) states that its an empowering tool with more potential than any other tool developed by mankind. Digital media is very much a double edged sword and has b oth positive and negative aspects to it. This essay will explore these different aspects and look to given a definitive answers as to whether the digital media and the internet has overall had a good or bad impact on broader culture and society. Positive impact on culture and society There are a significant number of ways in which the use of digital media has facilitated democracy and pluralism in worldwide society and culture. Supporters of this view include the likes of Goodwin, Jenkins and Burrows. The inventor of the World Wide Web, Sir Tim Berners-Lee, saw the internet as a tool that connected humanity. W ith 35 million people using the internet every day in the UK alone, he sees the internet as a platform for equal access to voice, opportunity and information, having originally been designed as a rebellion against hierarchy and authority. Wikipedia is definitely a product of the digital media that has had a positive impact on culture and society. Every month 65 million people use this site, with the original idea being that instead of information being handed down by experts and the elite above, it instead slowly emerges from the masses from below. This is a good example of the democratisation of society and culture that the digital media brings, as Wikipedia allow people to shape knowledge together on one platform. Twitter is a social media site that has had a strong positive effect on culture and society. This digital media platform can be used to campaign for fundamental freedoms in culture and society across the globe. For example Twitter was used to campaign for fundamental civil rights Kenya during the violence in the Kenyan election of 2008. Witnesses used Twitter to report the violence and corruption that the worlds media was not reporting accurately. Twitter represents a new form of democracy in society and culture, meaning that countries hierarchy in effect has flipped from vertical to horizontal. Another example can be found in Iran; when riots occurred the government banned world media from the area, however the public used Twitter to alert the world of what was occurring. Napster is a digital media site that advanced culture and society by undermining centuries of copyrighting and property. This site allowed music to be listened to for free, making it equally accessable and shared for free. This demonstrated digital media and the internets power to destroy established business models. While this site was completely illegal, the issue was that because the usage of this site became so widespread, lawsuits would have been needs for everyone between the ages of 18 and 30 at the time. As a result of Napster, 95% of all music exchange online in now unpaid for, showing how digital media has helped create added cultural equality and democracy over the last decade. YouTube is another strong example of how the digital media and the internet has had a positive effect on the global culture and society. YouTube was first created in 2005, and is now viewed 1 billion times a day, with 1/5 of all content on the internet itself being created by amateurs. The attraction to this site is that it provides and platform for self-promotion and takes control away from the middleman such as agents of publishers. These older hierarchies are still at large today, however their power is dwindling as they struggle to adapt to the digital medias democratisation and equalization effect on society and culture. Having first been created to protect the USA in the 1960s to link information between the government, the armed forces and institutions of science and universities, the internet has now become a de-centralizing power to the state. The internet is now seen as a threat to the state, de-centralizing power in single nations by merging their individual economies into a global economy. The rise of the digital media and the internet is accelerating globalisation which in turn provides new developments in culture that cross historic and traditional borders. Old centres of power are crumbling, meaning a huge scramble to fill the vacuum left behind. An example of this can be seen in Wikileaks, which allows people to anomalously publish information on governments such as classified US army documents on Guatanamo Bay, which helps challenge censorship. Suppressing information has become increasingly difficult, with the digital media and the internet helping make traditional censored countries suc h as China become far more democratic. When one part of the internet becomes controlled and colonised, a new frontier will always spring up in its place elsewhere. This certainly shows how the digital media benefits society and culture in a broad sense globally. Negative impact on culture and society There are many negative aspects that the rise of the internet and digital media has brought to culture and society. The digital media can be seen as a source of control and homogenisation, with supporters of this view including the likes of Schiller and Virilio. Around 25% of the global population has access to the internet, which can be seen to show another form of control by the MEDCs over LEDCs. It is in human nature to have the desire for both profit and control. If you can control what people believe in you control what people have access to, hence the internet and the digital media is a powerful tool that can create this control if harnessed by the appropriate people in society. The internet and digital media has allowed elite megabrands to gain power with no competition. The idealistic view (steming from 1970s hippie culture) that the world wide web should be a creative space where all people can share information for free, which went against those who wanted use the web as a place to buy and sell, use the web as a market place. Some people, such as Bill Gates, saw the internet as the biggest business opportunity ever. In 1995 Microsoft launched Internet Explorer and ended up with more than 90% of the market globablly. This is just an example of how the world dominated by just a handful of mega brands. In Britain 1/3 of the population has Facebook, Ebay has 21 million visits a month while Amazon get 16 million visits per month. There is one search engine (Google), one marketplace (Ebay), one bookshop (Amazon), one cinema (YouTube) and one social network (Facebook) that matter. This means there is a new massive wealth and power in the hands of a tiny elite t hanks to the internet. It is a huge historic anomolie in the fact that there are no competitors (Coke and Pepsi, Honda and Toyota)that would usually reflect a capitalist society. Hence this is a pure manifestation of way in which power works, landing itself to a very narrow oligarchy and elite in society. The digital media and the internet can be seen as a tool of control and oppression. Increasingly it mirrors hierarchy and inequalities with its originally idealistic beginnings fading over time. It is a powerful tool the state can use to access information and control the masses. An example of this is seen in China (one party state) that has 250 million of it population currently using the internet. Technology has helped drive Chinas economic growth and the government is now worried about their communist culture and society due to the rise of digital media and the internet. The government has employed 30,000 people to police the web full-time in China, developing a firewall around the country preventing many western media sites from entering and influencing public opinion. Their surveillance of social network sites is essential to their state control, hence the government has hired bloggers 300, 000 people to post communist support on digital media sites and blogs. The digital media can be seen to be narrowing identity. The internet can link and connect extremists, which has given them new tools of terrorism. Al Qaeda try to implement control through fear via the internet through hatefull messages and shocking images. This is very difficult to prevent as there is no central control centre of base on the internet. Digital media removes national borders and therefore these extremists have a virtual reality nation to spread their message. The internet and digital media can be see as eroding the concept of privacy. Private information is now exploited for highly targeted advertising and profit. For example Google gathers billions of search terms that help them sell highly targeted advertising. Its turned human curiosity into a goldmine, as Google now makes $200 per second for this scheme. Internet cookies now track our interests and website history. This can even Facebook, a company that increasingly uses technology to recognise pictures and send targeted adverts. Today, after purchasing an item, you are constantly emailed with updates. This is a manifestation of the attempt by big businesses and coorperations to colonise the online marketplace . It homogenises consumers with messages such as people who bought this also bought this. Almost without realising it, our search history is stored on a database of the companies that give us access to internet. Its suprising how much all the searches can be pieced together, and give a picture of who may have made these searchs. Hence this potentially could be used against individuals, as blackmail in the future. While this is an extreme view, this is a distinct possibility, and means corporations such as Google and Facebook have a huge amount of power they could utilize in the future. Analysis The positive aspects of digital media and the internet currently and always will outweigh the negatives aspects of digital media and the internet. It is irrational for anyone to claim that we were better off in an era of information poverty and un-empowered masses. One would rather have information overload than information poverty in society and culture. The internet is a true digital and electronic frontier where everyone is on his or her own; all manuscripts are accepted for publication, they remain in virtual print forever, and no one can tell writers what to do. The rise of the internet and digital media has empowered the masses and given everyone a platform on which to speak to the world. Of course, that doesnt necessarily mean all of the internet users will have something informed or valid to broadcast to across the world wide web. But such vast human empowerment is worth celebrating, despite its occasional downsides. Abundance in information is better than the old analog world of fewer choices and fewer voices. However, criticisms can be made against the internet and the digital media, as there are some very legitimate concerns regarding how the passing of the old order might leave society absent of some important cultural and order aspects. For example, one need not endorse bailouts for a dying newspaper industry to nonetheless worry about the important public service provided by investigative journalists: Who will take up those efforts if large media institutions go under because of digital disintermediation? The skeptics are also certainly correct that each of us should think about how to better balance new technologies and assimilate them into our lives and the lives of our families and communities. For example, children need to learn new digital literacy and cyber-citizenship skills to be savvy users of the world wide web. Conclusion This essay has critically assessed the impact that widespread use of digital media has on broader culture and society. For me, this was questioning whether digital media, the technological revolution and the rise of the internet can be seen as a blessing or a curse on culture and society. According to Castells (2002) who wrote a lot on this topic, new media technologies simultaneously reinforced relations of cultural capital, hierarchy and distinction, while enabling social movements to publicise campaigns and connect with distant others. Technology, the internet and the digital media has created unimaginable wealth yet also encouraged millions to work for nothing. Digital media Challenges authority yet allowed regimes to spy and censor as never before. The internet opens up new realms of knowledge and Al Gore (former vice president) states that its an empowering tool with more potential than any other tool developed by mankind. Digital media is very much a double edged sword and has both positive and negative aspects to it. This essay explored these different aspects and can now give a definitive answer as to whether digital media and the internet has overall had a good or bad impact on broader culture and society. Overall, the internet and the digital media is a positive phenomenon for humanity, society and culture. There are a significant number of ways in which the use of digital media has facilitated democracy and pluralism in worldwide society and culture. Strong examples in the physical form of digital media sites on the internet can be used to show this. Firstly Wikipedia is definitely a product of the digital media that has had a positive impact on culture and society. Secondly Twitter is a social media site that has had a strong positive effect on culture and society. Thirdly Napster is a digital media site that advanced culture and society by undermining centuries of copyrighting and property. YouTube is another strong example of how the digital media and the internet has had a positive effect on the global culture and society. These websites are contributing to rise of the digital media and the internet is accelerating globalisation which in turn provides new developments in culture that cross historic and traditional borders. The rise of the internet and digital media has empowered the masses and given everyone a platform on which to share information and dissolve old forms of hierarchy. However, to a smaller extent, the internet and digital media has caused a negative affect on society and culture. The digital media can be seen as a source of control and homogenisation by MEDCs over LEDCs. The internet and digital media has allowed elite megabrands to gain power with no competition. Again, the digital media and the internet can be seen as a tool of control and oppression. Increasingly it mirrors hierarchy and inequalities with its originally idealistic beginnings fading over time. The digital media can be seen to be narrowing identity while the internet can also link and connect extremists, which has given them new tools of terrorism. Finally, the internet and digital media can be see as eroding the concept of privacy. Private information is now exploited for highly targeted advertising and profit.

Statue of the Blessed Virgin :: Religion Prayers Irish Papers

Statue of the Blessed Virgin One of the best-kept secrets of Irish culture is the moving statues phenomenon that erupted in the mid-eighties all over the republic of Ireland. In August 1985, there were reports from the small town of Mountcollins that a statue of the Blessed Virgin had been seen to move. In the following months there were reports of the same phenomenon coming from small rural areas all over the country. Many people traveled to county Limerick to view the statue at Garryowen, following reports that some people had seen blood coming from one of the hands of Our Lady. The Rosary was recited several times each night as hundreds converged on the shrine. I myself went to once such gathering late at night at Mountcollins to see what was going on. The crowd chanted the Rosary over and over with their eyes glued to the statue of the Virgin Mary that was standing up in an alcove of a fake grotto similar to hundreds all over Ireland. It was dark and the statue had a spotlight on it. Behind the faithful, the re was a wood with the main road beyond. The Rosary recitals gradually gave way to "Mother of God, pray for us" that quickly became a rather feverish chanting of "Pray for us, pray for us, PRAY FOR US"! that got faster and more desperate as time pasted. Scenes like this sprang up nationwide with new sightings everyday. Everyone was talking about it at the pub, church or school and the media fanned the flames even more with daily coverage. Each person you spoke to who believed it was a true miracle, claimed a unique experience of the phenomena. Some said they saw the statue's hand move a bit or that a tear feel from her eye or most commonly the apparition of Christ's face upon the statue of Mary's face. The most skeptical about the whole rumpus was actually the clergy who found themselves dealing with a religious happening that was not within their control with impromptu prayer gatherings springing up everywhere. Their calls for caution in the media were generally disregarded by the members of the public caught up in the excitement. Some of this excitement began to turn a bit sour when rumors began to circulate that these miracles were actually dire omens of a coming apocalypse. It was reported in the papers that some woman claimed t o have had a vision that this was the beginning of a series of pre-apocalyptic portents that heralded the end of everything.

Properties of Enzymes and Competitive Inhibitors

Index Page Abstract†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦. †¦Ã¢â‚¬ ¦. 3 Introduction†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦. †¦Ã¢â‚¬ ¦.. 3 Materials and Chemicals used†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦. †¦.. 3 Procedures†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦ 4 Tables†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â ‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ 5-7 Results†¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦8 Discussion†¦. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ †¦Ã¢â‚¬ ¦8 Conclusion†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦Ã¢â‚¬ ¦. 8 Works Cited †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. 9 Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors, which affect enzyme activity.Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active site against substrates. Introduction: Cells have the ability to perform chemical reactions that at normal temperature outside the body proceed too slowly to support life. Cells are able to perform some reactions rapidly because they possess protein catalyst called enzymes. Enzymes are proteins that catalyze (i. . , increase the rates of) chemical reactions. Each enzyme has a unique globular shape, a small portion of which functions as an active site capable of binding to specific reactants or substrates. It was hypothesized that enzyme concentration, temperature, and inhibitors will affect the properties and abilities of the enzyme. Materials: 1Wax Marking Pens 150 ml Beakers 3 400 ml Beaker 1 container of parafilm 1 set of 20 spec tubes 1 regular test tube rack 1 small test tube rack 1 box Kimwipes Eye Droppers 1 thermometer 2-10ml Graduated Cylinders 1 Spectrophotometer 7  °C waterbath with test tube racks Solutions: 1 flasks of pH 7 buffered ONPG 1 flask of Lactose 8% 1 flask of pH 7 buffered 1 flasks of 8% beta galactosidase Procedure 1. Obtain five test tubes and label them (i. e. A, B, C, D, E) 2. Using a 10 ml graduated cylinder put: Note: It is very important to add enzyme last. 1 ml of pH 7 buffered ONPG + No Lactose 8%(0ml) +(1 ml pH buffer) + Enzyme (1ml) solutions into tube A. 0% Lactose. 3. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 25ml) +(. 75ml pH buffer) + Enzyme (1ml) so lutions into tube B. % Lactose. 4. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 5ml) +(. 5ml pH buffer) + Enzyme (1ml) solutions into tube C. 4% Lactose. 5. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (. 75ml) +(. 25ml pH buffer) + Enzyme (1ml) solutions into tube D. 6% Lactose. 6. Using a 10 ml graduated cylinder put: 1 ml of pH 7 buffered ONPG + Lactose 8% (1ml) +(0ml pH buffer) + Enzyme (1ml) solutions into tube E. 8% Lactose. 7. Cover each of the tubes with parafilm and place the tubes in the 37  °C waterbath for 30 minutes. . After 30 minutes, determine if the reaction has occurred in each tube, and notice change in color. 9. Test tube E acted as our control test tube because no competitive inhibitor was added. Lactose was the competitive inhibitor for this reaction into the test tube. Note: Because the result on steps 4 and 6 were not accurate for our particular experiment, steps 4 and 6 were performed twi ce. The following table and graph express the results after the measurements and mixing. Table 1. Measurements after mixing the solutions into the test tubes.Solutions| pH 7 Buffered ONPG (ml)| Lactose 8% (ml)| pH buffer (ml)| Enzyme B-Gal (ml)| Total amount of mls. | Test tube A| 1| 0| 1| 1| 3| Test tube B| 1| 0. 25| 0. 75| 1| 3| Test tube C| 1| 0. 5| 0. 5| 1| 3| Test tube D| 1| 0. 75| 0. 25| 1| 3| Test tube E| 1| 1| 0| 1| 3| This table represents the total amounts of each solution added to each test tube in order to get 3 mls for each test tube. This table is used only to represent how the result will look like. Graph 1. Measurements after mixing the solutions into the test tubes. This graph depicts the contents inside the test tubes after mixing the mentioned solutions.Measurement of O-nitrophenol. (ONPG) Although the appearance of yellow in the tubes indicated that O-nitrophenol was present, the color, alone, did not tell us how much was present. It was possible to measure the a mount of O-nitrophenol present by measuring the intensity of the yellow with a spectrophotometer. 1. The contents of the 5 tubes were poured into spec 20 tubes. The positions were labeled, but the spec tubes were left clear in order to have an accurate measurement absorbance. 2. Test tube E acted as the control tube for this, since that tube did not contain inhibitor.Note: Absorbance 420nm in this experiment will be a measure of the concentration of the O-nitrophenol molecules in each of the solutions. Using the Spectrophotometer The spectrophotometer was an instrument designed to measure the amount of light transmitted through solutions, or absorbed by substances in the solution. Light of a specific wavelength is emitted from a special bulb and passed through a tube containing a substance solution. The greater concentration of those particles; the greater the absorbance. It is very important to select the most appropriate wavelength of light for use.These procedures were followed i n order to set up the Spectrophotometer. 1. 420 nm was the wavelength to use in the inhibitor experiment lab designed because O-nitrophenol maximally absorbs a light at 420. 2. The Spectrophotometer was zeroed out with the control knob so that the needle reads 0% transmittance on the upper scale. 3. The control tube A was put in the holder, and the lid was closed. The light control knob was adjusted so that the needle could read 100% transmittance. 4. The control tube was removed from the holder. The lid was then closed noticing the needle again read 0% transmittance. 5.All other test tubes were placed into the Spectrophotometer and read as well. 6. Data for these results was recorded on the following table. Table 2. Effect of competitive inhibitor concentration lactose on the production of O-nitrophenol. Effect of Competitive Inhibitor Concentration on production of ONGP Product| Tube| Inhibitor Concentration| Intensity of yellow| Absorbance| ? moles of ONPG produced/30min | ? mole s of ONPG produced/min| A| 0%| ++++| 1. 55| 38. 75| 1. 291666667| B| 2%| +++| 0. 43| 107. 5| 3. 583333333| C| 4%| ++| 0. 13| 32. 5| 1. 083333333| D| 6%| +| 0. 02| 5| 0. 166666667| E| 8%| 0 | 0| 0| 0|Calculation of ? moles O-nitrophenol produced per minutes. Ex. Tube A: ? moles of ONPG produced/30min Absorbance/0. 004= ? moles of ONPG produced per 30min 0. 155 / 0. 004= 38. 75 ? moles Ex 2 Tube A: ? moles of ONPG produced/min ?moles of ONPG produced per 30min/ 30min 38. 75 /30=1. 291666667 ? moles of ONPG produced/min From the absorbance data that was measured the O-nitrophenol produced per minute was calculated. 1. Each ? mole of O-nitrophenol produced an absorbance of 0. 004. The absorbance measured was divided by 0. 004 to determine the number of ? moles produced during the experiment.The values were recorded in table 2, fifth column. 2. The measurements that were obtained in the fifth column were divided by 30(number of minutes left in the waterbath) to obtain the number of ? mol es of O-nitrophenol produced per minute. Graph 2. Absorbance measurements for inhibitor concentration lactose on the production of O-nitrophenol. Absorbance Absorbance Test Tubes Test Tubes Results According to the hypothesis that temperature, enzyme concentration, and concentration will affect the properties and functions of the enzymes. The hypothesis was supported because graph and tables express the change in absorbance, and ? oles produced. Discussion The tables were able to depict the result in order to get better and accurate results for this particular experiment. Measurements have to be performed with precaution, making sure the enzyme and the contents are mixed properly and at the same time. Conclusion Enzyme activity can be affected by other molecules. Inhibitors are molecules that decrease enzyme activity; activators are molecules that increase activity. Activity is also affected by temperature, chemical environment, change in pH, and the concentration of substrate.

The Transactional Model of Communication: An Equation

They're both positively welcoming the communication, they're both consenting artisans in the communication, and making predictions on the appearance of the approaching communication, they've both got positive expectations of the outcome. The conversation begins with Person A saying, â€Å"Hello, I'm Aaron†. Person B replies, â€Å"HI, I'm Betray'. They both smile to each other, but Aaron loses eye contact and looks up and down quickly at Botany's figure. Betray predicts that Aaron is judging her outer appearance which could convey that Aaron may be Judgmental in general or a misogynist that doesn't respect her.These are variables. These, along with other rabbles, determine the outcome of the session of communication. The personality variables will be put into brackets [ suggesting the most likely possibilities of the individual. Within these personality variables, there is always the hidden variable within, leaving the possibility open for an unmentioned or less-likely person ality option such as mental Illness. This assumptive variable will be labeled as ((X)). A# [Judgmental? Misogynistic? Socially X)? ] B# [Femaleness? Overly-sensitive? Accurately Observant?The equation has changed because Betray is no longer positively viewing this communication, she costive outcome. [A#] Aaron doesn't detect that Betray is now uncomfortable, so he is still undoubted his positive outcome. Betray is no longer so positive after her first impression of Aaron. Betray asks Aaron half-heartedly about the quality of his day. Aaron, unaware of Betrays perception, answers cordially with: â€Å"My day has been pretty good, and yours? † Judging by Aaron's casual and cordial response, he conveys that he would most likely welcome a similar response.Betray does not meet Aaron's expectations and she replies by saying, â€Å"It was alright earlier, but then I went to get my hair done ND it went by really slow 'cause I didn't have any good magazines to read†. Aaron is u nimpressed and somewhat deflated after realizing that the woman that he may have predicted as ‘intelligent' has proven otherwise. By Betray breaking one of Aaron's implicit social rules of replying with courtesy and simplicity, he has now made predictions about Betrays personality.He considers that she may be immature, air-headed, or simply uninteresting, and of course the hidden possibility (X). Aaron makes the predictions about Betray, and he now has a negative view on the communication. Betray on the other hand simply appreciates the few seconds that Aaron listened and this new impression has made up for the previous one. Betray once again positively views the communication. A# Judgmental? Misogynistic? Narcissistic? And/or (X)] (Aaron's prediction) (Betrays prediction) B# [Feminist? Overly-sensitive? Accurately observant? Air-headed?Immature? And/or (X)] Based on the actions and reactions of Aaron and Betray, their predictions of the outcome may change constantly or not at all throughout the entire communication process. The actual outcome is only determined once the transactions and the sections of their communication has ceased. It's like playing musical chairs; the game doesn't stop until the music stops. If Aaron and Betray end on a mutually negative prediction of the outcome, they may part ways and never speak again, completely content with going on without communicating with each other again.If the communication ends with Aaron feeling negatively and Betray feeling positively, Aaron may be content with not communicating with Betray again, but Betray may feel inclined to want to communicate with Aaron again, and vice versa. Variables thin the individual's personality will also continue changing as more and more is revealed, hidden, discarded, or assumed within the relationship. The truths will be proven through the individual's actions while the false assumptions are discarded. For example: Aaron and Betray meet a second time.Aaron takes Betray to dinner; this act from Aaron leads Betray to discard the possibility of misogyny because his sensitive and courteous actions disprove it. Misogyny is no longer within the brackets, because it no longer seems plausible to for Aaron to be a misogynist. Aaron and Betray are seated at their table and the waiter asks what they'd like to drink. Aaron asks for a glass of water. Betray asks for a very particular brand of wine and then inquires about its aging and production process. Betray has unknowingly impressed Aaron, and Aaron discards the assumption that wine.Those assumptions are removed from her brackets. On this second encounter, they both disproved at least one negative assumption with their actions. Aaron no longer seems misogynistic and insensitive, and Betray no longer seems air-headed or immature. These new assumptions are subject to be supported or disproved at NY point within their relationship, and this will always be the case. These assumptive variables are forever subje ct to change until the perceptions and the actions of the individuals cease.Infinitely, every possibility that could change the outcome of this communicative transaction has the power to do so, but that doesn't mean that it will. (X) is a hidden variable in every form. (X) used in brackets is to imply the possibility of hidden, unmentioned, unconsidered, possibilities about a person. (X) used in the broad equation, such as below, would imply the hidden variable of situational possibilities. Other people Joining the transaction, interruptions, outside conflicts, etc. [A#] +- [B#] (+/-)(X) The situational possibilities are determined by the actual physical setting of the communicative transaction. The situational possibilities also have the power to be positive or negative forces in a communicative transfer, therefore altering the outcome, For example: If the situational variable (X) were a car wreck, this particular event would most likely (probability also depending on variables) cu rb the subject matter, physically interrupt, or completely terminate the communicative renovations between the two instantly.The two people would probably not continue talking about their previous topic if a collision had Just occurred. One or both of the individuals may run to give aid or to find help, the other may stand there like a buffoon while the other one helps, therefore bringing forth assumptions, discarding false assumptions, and effecting the perceptions of the other person (or people) and vice versa. The absolute outcome can only be achieved once everything is said and done, no more changes can be made to the relationship or the perceptions between he communicators, and so what is presented is accepted as evidence of truth.The outcome is reality, and reality is perception. (X) will always be a variable to everything since it is impossible to prove, disprove, or even identify every single probability, therefore leaving some (regardless of percent) room for inaccuracy. No thing is ever -absolute- in the eyes of a human being, so the (X) variable will forever present itself in everything that we perceive. The only absolute, is that there are no absolutes. -? The Transactional Model of Communication: An Equation—